The Bioimaging Facility at IST Austria is hosting a demonstration of a Nikon Ti2-LAPP TIRF system on March 23rd-26th, 2021. The TIRF setup will be temporarily available at our Nikon Ti2-2 setup, in room i04.u1.015.
Specifications summary:
- TIRF
- Two independent TIRF units for sequential Multicolor TIRF or simultaneous 2 color TIRF
- Precise alignment of the critical angle per wavelength
- Easy TIRF calibration and visual confirmation of TIRF angle
- Gradation ND filter for a very even TIRF illumination
- Available Laser Lines for TIRF: 488, 561 and 640
- FRAP
- FRAP device for photobleaching and photoactivation/conversion experiments
- Free control of stimulation ROI(s) shape, size and number
- Power and wavelength adjustment for different ROI groups
- Available Laser Lines for FRAP Device: 405, 488, 561 and 640
- Possibility for simultaneous TIRF imaging and photobleaching
- Focus Perfected
- Real time focus correction with the Perfect Focus System (PFS)
- Cameras
- Up to two Hamamatsu ORCA Flash 4.0 cameras for simultaneous dual color imaging
- TIRF Objectives:
- CFI Apochromat TIRF 60XC Oil, NA 1.49, WD 0.16 – 0.07 mm
- CFI Apochromat TIRF 100XC Oil, NA 1.49, WD 0.16 – 0.09 mm
- CFI Plan Apochromat Lambda 100X Oil, NA 1.45, WD 0.13 mm
Demo-test sessions:
We offer morning and afternoon slots between March 23rd – 26th morning. Please see the following schedule:
| Mon 22nd | Tue 23rd | Wed 24th | Thu 25th | Fri 26th | |
|
Morning 10:00 – 12:30 |
testing | BIF | available |
Alexander JOHNSON (Friml group) (DM) |
Ekaterina KRASNOPEEVA (Guet group) (ND/DM) |
|
Afternoon 13:30 – 16:00 |
BIF (DM) |
available |
Philipp RADLER (Loose group) |
Lukasz Kowalski (Loose group) (DM)
|
Nikola ČANIGOVÁ (Sixt group) |
Requests for demo-slots:
Please provide the following information to bioimaging@ist.ac.at:
- Preferred time slot
- short description of your sample: fluorophores, FRAPPA,
- multi-angle adjust required
Information page: Nikon Ti2-LAPP TIRF system