The Imaging and Optics Facility (IOF) is developing continuously. On this page we provide an overview of the ongoing activities in our events calendar (current maintenance, workshops, equipment demonstrations, courses,.. ), and link to the archive of our weekly update newsletter. to previous teaching activities (courses & seminars) and equipment demonstration & workshops.
Events
pre-announcement:
Demonstration of the Nikon AX-R confocal microscope with N-SPARC detector
Target Group: Researchers who are interested in high efficiency advanced confocal microscope, with elaborate automation options to improve reproducibility, throughput and implement 3rd party hardware (micro-fluidics / -manipulators, etc,…) – as well as researchers who have an interest to transfer experiments from the ISTA/IOF Nikon machine park to confocal platform. - Fully motorized Ti2-E inverted Microscope: Including Perfect Focus System
- AXR Confocal Scanner: with large field of view for imaging ultra-fine details and Resonant Scanner with large field of view for fast and gentle imaging
- Laser Lines: 6 VIS laser lines: 405, 445, 488, 514, 561, 640 nm and NIR laser line: 730 nm
- Z-Piezo: 600µm Z-Piezo
- Tuneable Confocal Detector: 4 channel flexible tunable confocal detector for flexible imaging
- NSPARC Detector: for high resolution imaging
- Objectives: CFI Plan Apochromat Lambda D (4X, 10X, 20X, 40X, 60X Oil, 100X Oil) and CFI Plan Apochromat Lambda S 25X and 40X Silicone Oil objectives
- Illumination: CoolLED pE-300 White with DAPI, FITC, TRITC Filtercube for sample finding
- Software: NIS Version 6.10.01
- High Resolution Imaging: Diffraction-limited confocal imaging via pinhole and ISM-based high-resolution imaging via NSPARC
- Live Cell Imaging: Fast and gentle with resonant scanner and high-speed z-stacking with triggered Piezo
- Spectral Detection: Via tunable detectors
- High Throughput Applications: Well plates, tissue samples, multi-point, intelligent acquisition
- This prolonged system demonstration will be joined with an additional “Nikon NIS Automation” workshop planned for March 2025. This workshop is aimed to train researchers for autonomous use of software based automation and supervised implementation of 3rd party hardware, using Nikon JOBS and Macro. Attendees of this workshop training will benefit by being able to optimally set up advanced automation routines using the newest automation features from Nikon NIS.
- find more details about the Nikon AXR-confocal workshop here
- find more details about the Nikon SMART Microscopy workshop here
Over CW 5 & 6, several maintenances will take place in i04 which require the restriction of access to our Systems.
You can find here a schedule overview of which maintenance will take place when:
i04.U1.015:
- 27.01. 12:00 to 17:00 – ventilation maintenance – System use allowed, prolonged stays in room are prohibited, CO2 not available
- 28.01. 13:00 to 31.01. 13:00 – room deep cleaning – services interrupted
- 06.02. 7:00 to 12:00 – electrical maintenance – services interrupted
i04.U1.018 & 019:
- 28.01. – 7:00-14:00 – ventilation maintenance – System use allowed, prolonged stays in room are prohibited, CO2 not available
- 29.01. 13:00 – 03.02. 13:00 – room deep cleaning – services interrupted
- 06.02. 7:00 to 12:00 – electrical maintenance – services interrupted
i04.EG (Cytometry):
- 28.01. 12:00 to 17:00 – ventilation maintenance – no air circulation. System use allowed.
- 04.02. 7:00 to 10:00 – electrical maintenance – services interrupted
- 05.02. 7:00 to 10:00 – electrical maintenance – services interrupted
i04.O2 (Olympus TIRF):
- 28.01. 7:00 to 29.01. 16:00 – combined maintenance – services interrupted
i04.O3 (verticals):
- 28.01. 7:00 to 10:00 – electrical maintenance – services interrupted
- 31.01. 7:00 to 14:00 – ventilation maintenance – System use allowed, prolonged stays in room are prohibited, CO2 not available
*Exact dates and times might still underly slight changes depending on Maintenance progression.
Dear all,
Welcome to ISTA and thank you for your interest to start using laser equipment. This general laser safety training is meant for NEW scientists and staff that are planning to use laser based equipment and/or need access to a laser lab. New users of microscopes of the IOF machine park have to sign up for the IOF Newcomer training, where the laser safety to use microscopes is already included. This General laser safety training is planned for every first Monday of the month, at 15:00h and normally takes about 30 minutes
Request for “General Laser Safety Training”
This training is required by austrian law and will be held in person. When you sign up via email you will receive a room number for the next training.
Best regards,
The Laser Safety Office:
i: https://iof.pages.ist.ac.at/laser-safety
pre-announcement:
Demonstration of the CSR-Biotech Mu-SIM & MicroscopyX Finer (3 weeks)
Location: ISTA Klosterneuburg, Austria
Target Group: Researchers who are interested in high resolution imaging for prolonged times
- 100X
- 60X oil objects,
- 60X water
- 20X air
Seminars:
- MI-SIM
- MicroscopyX Finer
Schedule: book a slot
Location: ISTA Klosterneuburg, Austria
Seminar Room: T.b.d.
System Specification:
- 100X
- 60X oil objects,
- 60X water
- 20X air
Schedule: book a slot
Image Analysis open-doors and Workstations maintenance
Struggling with image analysis, image acquisition or anything related to imaging or IOF instrumentation?
Feel free to stop by in rooms I01.O3.052a/b and ask your questions, doubts or advices at our image analysis open-doors! Now every 3rd Wednesday of the month.
IOF Team
pre-announcement:
Demonstration of the Nikon AX-R confocal microscope with N-SPARK detector
Location: ISTA Klosterneuburg, Austria
Target Group: Researchers who are interested in improving the efficiency advanced ‘SMART’ microscope, by implementation of advanced automation routines that integrate real-time analysis to improve reproducibility, throughput and learn about the options to steer 3rd party hardware .
This workshop is aimed to train researchers for autonomous use of software based automation and supervised implementation of 3rd party hardware, using Nikon JOBS and Macro. Attendees of this workshop training will benefit by being able to optimally set up advanced automation routines using the newest automation features from Nikon NIS.
Schedule: request a slot
Dear all,
Welcome to ISTA and thank you for your interest to start using laser equipment. This general laser safety training is meant for NEW scientists and staff that are planning to use laser based equipment and/or need access to a laser lab. New users of microscopes of the IOF machine park have to sign up for the IOF Newcomer training, where the laser safety to use microscopes is already included. This General laser safety training is planned for every first Monday of the month, at 15:00h and normally takes about 30 minutes
Request for “General Laser Safety Training”
This training is required by austrian law and will be held in person. When you sign up via email you will receive a room number for the next training.
Best regards,
The Laser Safety Office:
i: https://iof.pages.ist.ac.at/laser-safety
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