Leica Stellaris 5 (WLL, TauSense, DPA)

Location:

room I04.U1.019

Imaging modalities/features:

  • Confocal 
  • time gated acquisition
  • lifetime imaging (Tau Sense)
  • spectral unmixing
  • excitation Λ / emission λ wavelength scans

Specification:

  • DMi8 CS inverted research microscope
  • Motorized Scanning stage
  • Super Z Galvo Stage (Maximal travel range in z: 1500 μm, Minimal step size: 20 nm)
  • Resonance scanner (8 MHz)

Light sources:

  • Leica LED3 light source
  • 405, 448, 488 and 561 laser lines
  • White Light Laser (485  –    685 nm) Supercontinuum pulsed laser (max. 78 MHz): tunable in steps of 1 nm –  allows simultaneous use of up to 8 laser lines from UV to IR (8 channel AOTF: rapid modulation of laser intensity, particularly for ROI)

Filter for visual examination:

  • DAPI: Excitation: BP 405/60 Emission: BP 470/40
  • GFP: Excitation: BP 470/40 Emission: BP 525/50
  • RHOD: Excitation: BP 546/10 Emission: LP 590

Objectives – positions:

  1. HC PL APO 10x/0.40 CS2, WD 2.56 mm – no immersion
  2. HC PL APO 20x/0.75 CS2, WD 0.62mm – no immersion
  3. HC PL APO 63x/1.4, WD 0.16 mm – oil immersion
  4. HC PL APO 40x/1,1 W CORR CS2 , WD 0.65mm – water immersion

EXTRA high-end objectives are available via Automated Rental System:

  • HC PL APO 20x / 0.75 MI (# 15506343), WD 0.67 mm – multi-immersion (CS2): water, glycerine and immersion oil

Detectors: 

  • 3x HyD S detectors (410 – 850 nm, tunable detection with 1 nm step, min. range = 5nm)

Software: LAS X Stellaris (including TauSense)

Modules:

  • LAS X Live Data Mode
  • LAS X Dye Finder
  • LAS X 3D Visualisation
  • LAS X MicroLab: wizards for setup and analysis of FRAP, FLIM and FRET

System manual and additional material is available on SeaFile (login required).

Computer (CUDA Workstation):

  • Intel Xeon Gold 6244 processor (8 cores / 16 threads)
  • 96 GB working memory
  • Nvidia Quadro RTX 5000 graphics card with 16 GB graphic card memory and
    3072 CUDA cores
  • Fast 256 GB SATA SSD system drive (Windows, LAS X), very fast 2 TB M.2 SSD
    hard drive for temporary storage of data (transient data container of LAS X, not
    for the user) and normal 6 TB HDD drive for storage of data.

Contact