Zeiss LSM-04 (800inv_Airyscan)

Location: I04.U1.018 – book in PPMS  – 4h limit in core hours

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This confocal microscope with Airyscan: well suited for life imaging: 

Axio Observer.Z1, Inverted LSM800 confocal microscope + Airyscan, suited for live imaging: equipped with heating chamber, CO2 mixer,  ZEN 3.8 with experiment designer,macro-enviroment (OAD) and ZEN Connect.

Overnight acquisitions: Please reboot system before and start system in DEBUG MODE (+ screenshot script): do not forget to SAVE the debug log-file in case of a crash!!

Sample conditioning:

  • Heating chamber
  • CO2 mixer + humidifier

Light sources:

  • Laser lines 405, 488, 561, 640 nm
  • CoolLED p300 SB light
  • ESID LED

Detectors: 3 channels simultaneously (more sequentially)

  • 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
  • 1 x Airyscan detector – optimized for 63x. Can also be used as 3rd GaAsP detector!
  • 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

  • Motorized stage for multiple position imaging
  • Multi-slide holder, dish holder, single slide holder

Objectives (M27 thread):

  1. Plan-Apochromat 10x/0.45, WD=2.1 mm (420640-9900-000)
  2. Plan APOCHROMAT 20x/0.8 , WD=0.55 mm (D=0.17 mm) (420650-9901)
  3. Plan-Apochromat 40x/1.2 Water
  4. Plan-APOCHROMAT 63x/1.4 Oil (420782-9900-799)
  5. Plan-Apochromat 100x / NA 1.4 Oil  DIC

Software: Zen 3.8

  1. ZEN Module Experiment Designer Hardware
  2. ZEN Module Tiles & Positions
  3. ZEN Macro Editor

Computer: HP Z6

  • Intel® Xeon™ Gold 5122 v3 CPU @ 3.6GHz + 3.59 GHz
  • 64GB RAM
  • NVIDIA Quadro P400 (8Gb)
  • 8 TB data drive

Contacts person:

  • iof@ist.ac.at

System manual and additional material is available on SeaFile (login required).