Zeiss LSM-09 (880up_Airyscan)

Confocal scanner head for 3 descanned spectral channels, climate chamber

Location: Moonstone Building, room i24.U1.031 / wing B3 / compartment 3

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Specification: Zeiss Axio Examiner Z1 upright microscope

Detectors:

  1. 1 x GaAsP PMT
  2. 2 x multi alkali PMTs
  3. 1 x transmitted light (T-PMT) detector
  4. 1 x AiryScan super-resolution detector

Illumination / excitation:

  1. Argon laser lines: 458nm, 488nm, 514nm
  2. Violet (405nm)
  3. green (561nm) and red (633nm) Diode lasers
  4. CoolLED lamp (visual inspection)
  5. Halogen TL (visual inspection)

Objectives:

1: Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm) (mounted pos.1)
2: Plan-Apochromat 20x / NA 0.8 Air (mounted pos.2)
3: Plan-Apochromat 20x / NA 1.0 Water DIC (objective description) (mounted pos.3)

4: Plan-Apochromat 40x / NA 1.4 Oil (mounted pos.4)

  • Available not mounted:

5: Plan-Apochromat 63x / NA 1.2 Water

6: Plan-Apochromat 40x / NA 1.2 Water

Modalities: ZEN black v2.3

  1. Fluorescence Correlation Spectroscopy (FCS) option.
  2. ZEN Module:  Experiment Designer: time series, multi-time series, Z-stacks, tiles/multiposition images and channels in any order
  3. ZEN Module: Tiles & Positions: automatic scanning of predefined sample areas, plus acquisition of images according to a position list. Supports focus correction maps*, stitching and shading correction.
    Compatible with software autofocus.

Computer: HP Z820 Workstation,

  • 4 x 4GB RAM,
  • 128 GB SSD,
  • 2 x 2 TB SATA 7200 upm (RAID)

System manual and additional material is available on SeaFile (login required).

Laser safety: https://ppms.ist.ac.at/vdoc/?pf=2&docid=50