The Imaging & Optics Facility provides a number of non-charged devices for sample preparation or checking, as well as experimental accessories. These resources do not require a system training, but need to be booked in PPMS nevertheless. An introduction to newcomers is required before any instrumentation can be used.
Zeiss Axio Imager
Location (I06.U1.403): book in PPMS
- Manual stage
- Light sources: halogen lamp +CoolLED p300 SB light
- Detectors: monochromatic camera
- Software: Zen 2.1
- Objectives (M27 thread):
- Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
- Plan APOCHROMAT 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
- empty
- Plan-Apochromat 40x/1.2 Water, WD=0.28 mm
- Plan APOCHROMAT 20x/0.5 dipping (420650-9901), WD=0.22mm
Leica M125FC stereo microscope
Location: (room i04.U1.405): book in PPMS
- 0.8-10x Magnification, Top illumination; No fluorescence
- System for sample preparation and mounting – lightsheet
- Temporally located in room i04.U1.019
Leica M165FC stereo microscope + Orca R2 camera
Location: (room i04.U1.019): book in PPMS
- Different illumination option in light-base
- CoolLED p100 LED
- Filters-sets for DAPI (Ex:436/20 Em:480/40), GFP1 (Ex:425/60 Em:505/40), GFP2 (Ex:480/40 Em:510LP), RFP (Ex:546/10 Em:570LP)
- Hamamatsu Orca Flash R2 camera (6.45 x 6.45 um pixel size)
Leica M205 FCA stereo microscope + Orca R2 camera
Location: (room i24.U1.031): book in PPMS
- Objective Planapo 1.0x M-series.0x M-se
- Different illumination option in light-base
- CoolLED pE300 SB LED
- Filters-sets for DAPI (Ex:350/50 Em:460/50), GFP (Ex:470/40 Em:525/50), RFP (Ex:546/10 Em:590LP)
- Hamamatsu Orca Flash R2 camera (6.45 x 6.45 um pixel size)
Olympus SZX16 Stereo - 0.5x + Camera (i04/BFB)
Location: room i04.U1.015 book in PPMS
- Acquisition software: Cell Sense Entry
- 0.5x objective + camera
- Pixel size: 12.50µm/pixel (at 1x magnification)