Location: i04.U1.015
Widefield fluorescent microscope with incubation chamber and CO2 mixer available
Specifications:
- Timelapse option, motorized stage
- Perfect Focus System (PFS)
- DS-Qi2 monochrome camera
- DIC imaging with 10x, 20x and 40x objectives
- Automated Water Immersion Dispensing System
- Nikon JOBS software: automation of acquisition
Objectives:
- Plan Fluor 4x/0.2 air (MRH00041)
- Plan Fluor 10x/0.3 DIC 1 N1 air PFS (MRH00101)
- Plan Apo λ 20x/0.75 DIC 1 air PFS (MRD00205)
- Plan Apo λ 40x/0.95 DIC air PFS (MRD00405)
- APO LWD λS 40x / 1.15 Water 0.60 mm (MRD77410) – €€€
Filter sets:
- Single band pass filters: CFP, DAPI, GFP, mCherryC, Cy5, Cy7
- 3-band filter: CFP, YFP, & mCherry
- 5-band filter: DAPI, FITC, TRITC, Cy5 & Cy7
LUMENCOR SPECTRA III LED light source with flexible excitation band selection:
-
- Violet 385nm (390/22),
- Blue 440nm (440/20)
- Cyan 488nm (475/28)
- Teal 514nm (511/16)
- Green 561nm (555/28)
- Yellow 594nm (575/25)
- Red 640nm (635/22)
- IR 730nm (747/11)
System manual and additional material is available on SeaFile (login required).
Resources:
To open ND2-files in FIJI: please go to Plugins/Bio-Formats/Bio-Formats Plugins Configuration under Formats you have to find Nikon ND2 (it is alphabetically sorted) and disable Use chunkmap table.
In case this doesn’t work, please try to enable Nikon’s reader or different combinations of the last 2 check boxes
Contact: Imaging & Optics Facility