Leica SP8 inv (Argon, WLL, FALCON)

Location: I04.U1.015

Imaging modalities/features:

  • Confocal + real-time deconvolution (Lightning)
  • time gated acquisition
  • lifetime imaging
  • spectral unmixing
  • excitation Λ / emission λ wavelength scans

Specification:

  • DMi8CEL Advanced inverted Research microscope
  • TCS SP8 FALCON
  • Motorised Scanning stage
  • Super Z Galvo Stage Type H (Maximal travel range in z: 1500 μm Minimal step size: 20 nm)

Light sources:

  • Lumencor SOLA­ SM II, LED Light source
  • 405 nm diode laser CW: DMOD (50 mW)
  • Argon laser CW, lines: 458, 476, 488, 496, 514
  • 561 nm diode laser CW (no power at the moment, will be exchanged)
  • 633 nm diode laser CW
  • White Light Laser (470  –    670 nm) Supercontinuum pulsed laser (max. 80 MHz): tunable in steps of 1 nm –  allows simultaneous use of up to 8 laser lines from UV to IR (8 channel AOTF: rapid modulation of laser intensity, particularly for ROI)

Filter for visual examination:

  • DAPI: Excitation: BP 350/50 Emission: BP 460/50
  • FITC: Excitation: BP 480/40 Emission: BP 527/30
  • RHOD: Excitation: BP 546/10 Emission: BP 585/40

Objectives – positions:

  1. HC PL Fluotar 10x/0.30, WD 11.0mm – no immersion
  2. HC PL APO 20x/0.75 CS, WD 0.62mm – no immersion
  3. HC PL APO 40x/1.10 W CORR CS2, WD 0.65mm – water immersion (Correction collar for use with 0.14­ – 0.18 mm coverglass)
  4. HC PL APO 63x/1.30  CORR CS, WD 0.30mm – glycerol immersion (Correction collar for use with coverglass (0.14­0.19 mm)

EXTRA high-end objectives are available via Automated Rental System:

  • HC PL APO 20x / 0.75 MI (# 15506343), WD 0.67 mm – multi-immersion (CS2): water, glycerine and immersion oil

Detection Filters:

  • ­NF 445
  • NF 488
  • ­NF 514
  • NF 458/514
  • NF 488/561/633 ­
  • NF 594
  • NF 445/594

Detectors:

  1. PMT
  2. HyD2  SMD: specially selected for Falcon FLIM
  3. PMT
  4. HyD SP GaAsP­ Detector for gated imaging
  5. HyD SP GaAsP­ Detector for gated imaging
  6. Transmitted light detector (TLD)

Computer (CUDA Workstation):

  • 2x Intel 4­core Xeon E5­2637 V4 3.5 GHz
  • 128 GB RAM
  • NVIDIA Quadro GP100 16GB Reference GPU (3584 cores) ­
  • 256 GB SATA SSD
  • 2x 1TB SSD RAID 0
  • 5x 3TByte SATA hard disk drive, RAID5

Software: LAS X 2.5.7.23225

Modules:

  • LAS X Live Data Mode
  • LAS X Dye Finder
  • LAS X 3D Visualisation
  • LAS X MicroLab: wizards for setup and analysis of FRAP, FLIM and FRET
  • LAS X LIGHTNING Expert: Confocal Super­ Resolution

System manual and additional material is available on SeaFile (login required).

Contact persons: