This Laser Scanning Microscope (LSM) is an confocal platform that is explicitly specialized for advanced imaging applications for the analysis of Molecular Dynamics; Molecule concentrations; Superresolution microscopy (Airyscan 2) and Spectral analysis of complex samples (>5 channels). Additional trainings are required to be able to make use of the different applications.
Location: room I06.U1.403
Specification: Zeiss Axio Observer 7 inverted microscope, with Definite Focus 3
- 1x Spectral Detector: 32 channel GaASP
- 2x GaAsP PMT
- 1x AiryScan2: super-resolution detector + multiplex option (up to 8x parallelization ) and Dynamic profiler
- 1x transmitted light (T-PMT) detector
- Axiocam 305 monochrome Camera (2464 x 2056 pixels; 3.5 micon2 / pixel)
- Automated Piezo stage (XY) +
Software & Modules: Zen Blue 3.10
- Spectral Imaging
- Reflected fluorescence illumination
- Advanced Acquisition: z-stacks; tiles;
- ZEN Toolkit Smart Acquisition: Experiment Designer; Guided Acquisition; Experiment Feedback; AI sample finder
- ZEN Toolkit 2D and 3D
- ZEN Connect
- ZEN LSM Plus deconvolution
- Airyscan multiplex and deconvolution
- ZEN Dynamic Profiler Advanced: molecular characteristics for FCS; flow analysis (+ directionality)
- ZEN FCS and FCCS: Advanced Fluorescent Correlation Spectroscopy
Illumination / excitation:
- X-Cite Xylis LED, with peaks at 385nm, 430nm, 474nm, 545nm, 650nm and 735nm
- Laser lines: 405nm, 445nm, 488nm, 514nm, 561nm, 639nm
- Halogen Transmitted light (visual examination)
Sample conditioning:
- Temperature chamber
- Gas Incubation system for CO2 + O2 available from Ibidi GmbH
Objectives:
- EC Plan-Neofluar 2.5x/0.085 M27 (WD 8.8 mm) – sample finder
- Plan-Apochromat 10x /0.45 air M27 (WD 2.0 mm)
- Plan-Apochromat 40x/1.4 Oil DIC M27 (WD 0.13 mm)
- C-Apochromat 63x / 1.2 Water Autocorr FCS (WD 0,28)
PC: HP Z8 G5 workstation
- Intel® Xeon® CPU W7-3455 @ 2.5GHz
- 80GB RAM
- NVIDIA RTX A5000
- 10TB data drive + 8Tb SSD
System manual and additional material is available on SeaFile (login required).