The Bioimaging Facility at IST Austria is hosting a demonstration of a Nikon N-SIM system from October 4th – 20th, 2021.
The N-SIM S Super Resolution Microscope is a unique high-speed structured illumination system that achieves acquisition speeds of up to 15 fps, enabling fast biological processes to be captured at twice the spatial resolution of conventional light microscopes (up to 115nm in XY). Combining the N-SIM S and a confocal microscope gives you the flexibility to select a location in the confocal image and switch to super-resolution to view the desired part of the location in minute detail. (source)
Technical details of the Demo system
- Microscope:
- Ti2-E Inverted Microscope with motorized stage, Z-Piezo Insert and Perfect Focus System
- Lasers:
- 405 nm/445 nm/488 nm/561 nm/647 nm
- Objectives:
- CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49)
- includes an auto correction collar
- CFI Plan Apochromat Lambda 60XC (NA 0.95)
- CFI Plan Apochromat Lambda 40XC (NA 0.95)
- CFI SR HP Apochromat TIRF 100XAC Oil (NA 1.49)
- Cameras:
- 2 x Hamamatsu Orca Fusion sCMOS cameras (Hamamatsu Photonics K.K.)
- Hamamatsu Gemini-2C Beamsplitter
- Incubation System
- SIM Imaging modes
- TIRF-SIM
- 2D-SIM
- 3D-SIM (Reconstruction methods: slice, stack)
- Multi-color imaging
- Simultaneous multi-color imaging of two colors
- Image acquisition time Up to 15 fps (TIRF-SIM/2D-SIM, 2 ms exposure time)
- Lateral resolution (FWHM of beads in xy):
- 115 nm in 3D-SIM mode,
- 86 nm in TIRF-SIM mode
- Axial resolution (FWHM of beads in z):
- 269 nm in 3D-SIM mode
Specifications summary:
-
Lateral resolution (FWHM of beads in xy) 115 nm*1 in 3D-SIM mode, 86 nm*2 in TIRF-SIM mode Axial resolution (FWHM of beads in z) 269 nm*1 in 3D-SIM mode Image acquisition time Up to 15 fps (TIRF-SIM/2D-SIM, 2 msec exposure time) Reconstructed image size 1024 x 1024 pixels, 2048 x 2048 pixels
Demo-test sessions:
We offer morning and afternoon slots starting from October 4th to 8th (week 1), October 11th to 15th (week 2) and from October 18th to 20th (week 3). The N-SIM system will stay at IST until the 20th of October in our room in the multi-purpose hall. We will have a super resolution application specialist from Nikon on site from October 4th to October 8th, 2021. In the second Demo week from 11th to 15th of October 2021, the system will be operated by BIF members. External scientists are welcome to try the system.
| 1st week | Mo, October 4th | Tue, October 5th | Wed, October 6th | Thu, October 7th | Fri, October 8th |
| morning 9 to 12 am | BIF | C. Gianinni; grad school | NIKON | BIF | |
| afternoon 1 to 4 pm | BIF | A.Johnson; Friml group | M.Javoor; Schur group | BIF |
| 2nd week | Mo, October 11th | Tue, October 12th | Wed, October 13th | Thu, October 14th | Fri, October 15th |
| morning 9 to 12 am | NIKON tech | NIKON tech | |||
| afternoon 1 to 4 pm | NIKON tech | NIKON tech | N.Canigova; Sixt group | Marko Kojic, Loose grp (cy3 & cy5 on lipid bilayers) |
| 3rd week | Mo, October 18th | Tue, October 19th | Wed, October 20th |
| morning 9 to 12 am | Y.Vallis; De Bono group | Uni Wien (Köhler-grp) | |
| afternoon 1 to 4 pm | Suyash NAIK; Heisenberg grp GFP & mCherry | Uni Wien (Köhler-grp) | Philipp Radler, Loose grp |
Requests for demo-slots:
Please provide the following information to bioimaging@ist.ac.at:
- Preferred time slot
- short description of your sample: fluorophores, module, aim
Sample preparation
To prepare/mount your samples please only use 1.5H precision glass coverslips (170 µm +/- 5 µm) (see ref. 1-2 for examples) or high precision (#1.5H) glass bottom dishes (see ref. 3):
Location: Multi Purose Hall IST Austria Campus, 3400 Kloserneuburg
Information page: Nikon Ti2-LAPP TIRF system