Automated upright widefield fluorescent microscope with Zeiss Zen Connect capabilities.
Description
Location: i04.U1.018
Specifications:
- Timelapse, tilescan, motorized stage
- Zeiss Axiocam 202mono monochrome camera
- Upright Imaging with air or dipping objectives
- Zen Connect: multi-modal combination of different Zeiss machines
Objectives:
- Zeiss Plan-Apochromat 5x/0.16 AIR (420630-9900), working distance = 12.1 mm
- Zeiss Plan-Apochromat 10x/0.45 AIR (420640-9900), working distance = 2 mm
- Zeiss Plan-Apochromat 20x/0.8 AIR (420650-9901), working distance = 0.55 mm
- Zeiss Plan-Apochromat 20x/1.0 WATER (421452-9880), working distance = 1.7 mm
(this dipping objective is designed for the use of physiological salt solution as an immersion medium)
Filter sets:
- DAPI – BP335-383, BS 395, BP 420-470 (Zeiss, filter set 49)
- eGFP – BP450-490, BS 495, BP500-550 (Zeiss, filter set 38 HE)
- mRFP – BP 550-585, BS590, BP 600-690 (Zeiss, filter set 63 HE)
CoolLED pE300 light source
Transmission light source
System manual and additional material is available on SeaFile (login required).
Additional information
| Building | i04 |
|---|---|
| Camera / Detector | CMOS |
| Fluorescent Lightsource | LED: 385-395nm, LED: 505-515nm, LED: 545-555nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Automated Stage |
| Technology / Application | Time-lapse recording, Multi-position, In-vivo imaging |
