Automated widefield microscope for brighfield, phase contrast & fluorecence imaging complex, as well as complex automation routines
Description
Automated Widefield Fluorescent Microscope
Location: i23.U1.015 – book in ppms
Specifications:
- Motorized stage, with multi-position option
- Timelapse option
- Colour and monochrome cameras (see manual for pixel size)
- Phase contrast imaging with 10x, 20x, 60x oil, 100x oil objectives
Objectives:
- 4x/0.2 air
- 10x/0.3 PH1 air
- 20x/0.5 PH1 air
- CFI Plan Apo Lambda 40XC/0.95Air – WD: 0.25–0.16 mm
- 60x/1.4 PH3 oil
- 100x/1.45 PH3 oil
Emission filters:
-
- DAPI (LP 409, BP 447/60)
- Piston GFP (LP 495, BP 520/35)
- TexasRed HYQ=mCherry (LP 596, BP 641/75)
CoolLED pE300 White fluorescence lightsource
-
- Violet (390/18)
- Blue (438/24)
- Green (549/15) (always in combination with Yellow)
System manual and additional material is available on SeaFile (login required).
Contact: Imaging & Optics Facility
Additional information
| Building | i23 |
|---|---|
| Camera / Detector | CCD, Color CMOS |
| Fluorescent Lightsource | LED: 385-395nm, LED: 430-450nm, LED: 545-555nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Illumination: phase contrast, Incubation: heating stage, Automated Stage, Focus Stabilization |
| Technology / Application | Time-lapse recording, Multi-position |
