Flow Cytometry Service

For Flow Cytometry – Fluorescent Assisted Cell Sorting (FACS) – services, we offer instrument training, assisted sessions and project based work-flow optimization. In addition, we offer support on all available Flow cytometry Analysis Software applications that are available on our Cyto-WS analysis workstation. Service can be requested via the request forms in PPMS.

  • Instrument Trainings: instument training for obtaining user rights
  • Assistance: support for optimization of experiment during a single session
  • Project based work-flow optimization: multi-day application optimization (projects discussion and PI approval required)

Communication

Please send your inquiries to cytometry@ist.ac.at →  the cytometry team will reply to your ticket query!

Available instrumentation:

– BC Cytoflex XL – Cell Analyser

– BD FACS Aria III  – Cell Sorter

– Sony SH800SFP – Cell Sorter

– Cyto WS – Analysis Workstation

 

Available Cytometry Analysis software:

For the analysis of Flow Cytometry data, we provide various cytometry analysis software applications on our analysis workstation:

CytExpert

CytExpert is the Software that runs BDs CytoFlex line of Cytometers. It can also be used to do basic statistics of data coming from CytoFlex and features an analysis mode that allows comparison of samples. Further information can be found → here

Flowing Software

Flowing Software is a free cytometry data analysis programm from University of Turku. It allows an intuitive flow-chart like set-up of analysis routines. Further information can be found → here

Cytoflow

Cytoflow is a free cytometry data analysis programm that allows advanced analysis routines including complex statistics and model-fitting. Further information can be found → here

Kaluza

Kaluza (Beckman Coulter)  can be used in the same way as ‘FlowJo Software’ for analysis of any of your .fcs files. Detailed manual on Kaluza can be found here.

FlowJo

FlowJo is one of the most common Flow Cytometry Analysis Sotware (FlowJo v10.8.1 see functions).

You can reach the plugins through the Workspace menu. Note, that some have to be applied to the whole workspace through the ‘Add Workspace Plugin’ option in order to work, and for some, you have to select the appropriate tubes first, and then apply the plugin.

Further plugins are available on FlowJo Exchange – reach out if you’d like to have them installed.