The vertical point scanning confocal microscope and root-tracker is a unique device that allows to image and track multiple samples / plant roots over a prolonged time in an upright position
Description
Location: room I04.O3.014 (BFB)
- 2 MA-PMT detectors: 2 channels simultaneously, more sequentially
- 1 AiryScan array detector, also usable as a 3rd GaAsP detector in confocal mode
- Multiple position imaging
- Gravitation manipulation (rotation stage)
- (Root-)tracker (max 8 positions & 128 time points each)
Laser lines:
- 405 nm, 488 nm, 561 nm, 640 nm
Objectives (M27 thread)
- Plan-Apochromat 10x/0.45 , WD=2.1 mm – (420640-9900-000)
- Plan APOCHROMAT 20x/0.8 , WD=0.55 mm (D=0.17 mm) – (420650-9902-000)
- C-Apochromat 63x/1.2 Water, WD=0.28mm (CG=0.17mm) (421787-9970-799)
Software: Zen 3.8
System manual and additional material is available on SeaFile (login required).
- FIJI script to convert the image-sequence from tracker to concatenated files per position can be found here
- More details about the system can be found here: https://elifesciences.org/articles/26792
Additional information
| Building | i04 |
|---|---|
| Camera / Detector | ESID, MA-PMT, Airyscan (SR) |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 445±5nm, Laser: 488±5 nm, Laser: 561±5 nm, Laser: 640±5 nm, LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Automated Stage, Microfluidics |
| Technology / Application | FRAP, FRET, Time-lapse recording, Multi-position, Sample Stabilization / (Root-)Tracker, In-vivo imaging, Photoactivation / -conversion, SR: Airyscan (140nm), Super Resolution (SR) |
