This Laser Scanning Microscope (LSM) is an confocal platform that is explicitly specialized for advanced imaging applications for the analysis of Molecular Dynamics; Molecule concentrations; Super Resolution microscopy (Airyscan 2) and Spectral analysis of complex samples (>5 channels). Additional trainings are required to be able to make use of the different applications.
Description
Location: I06.U1.403
Specification: Zeiss Axio Observer 7 inverted microscope, with Definite Focus 3
- 1x Spectral Detector: 32 channel GaASP
- 2x GaAsP PMT
- 1x AiryScan2: super-resolution detector + multiplex option (up to 8x parallelization ) and Dynamic profiler
- 1x transmitted light (T-PMT) detector
- Axiocam 305 monochrome Camera (2464 x 2056 pixels; 3.5 micon2 / pixel)
- Automated Piezo stage (XY) +
Software & Modules: Zen Blue 3.10
- Spectral Imaging
- Reflected fluorescence illumination
- Advanced Acquisition: z-stacks; tiles;
- ZEN Toolkit Smart Acquisition: Experiment Designer; Guided Acquisition; Experiment Feedback; AI sample finder
- ZEN Toolkit 2D and 3D
- ZEN Connect
- ZEN LSM Plus deconvolution
- Airyscan multiplex and deconvolution
- ZEN Dynamic Profiler Advanced: molecular characteristics for FCS; flow analysis (+ directionality)
- ZEN FCS and FCCS: Advanced Fluorescent Correlation Spectroscopy
Illumination / excitation:
- X-Cite Xylis LED, with peaks at 385nm, 430nm, 474nm, 545nm, 650nm and 735nm
- Laser lines: 405nm, 445nm, 488nm, 514nm, 561nm, 639nm
- Halogen Transmitted light (visual examination)
Sample conditioning:
- Temperature chamber
- Gas Incubation system for CO2 + O2 available from Ibidi GmbH
Objectives (M27 thread)
- EC Plan-Neofluar 2.5x / NA0.085 / WD 8.8 mm – Air (sample finder)
- Plan-Apochromat 10x / NA 0.45 / WD 2.0 mm – Air
- Plan-Apochromat 20x / NA 0.8 / WD 0.55 mm – Air
- Sample Finder Mirror (SF)
- Plan-Apochromat 40x / NA1.3 / WD 0.13 mm DIC – Oil
- C-Apochromat 63x / NA1.2 / WD 0,28 Autocorr FCS – Water
PC: HP Z8 G5 workstation
- Intel® Xeon® CPU W7-3455 @ 2.5GHz
- 80GB RAM
- NVIDIA RTX A5000
- 10TB data drive + 8Tb SSD
Additional information
| Building | i06 |
|---|---|
| Camera / Detector | CCD, CMOS, GaAsP-PMT, Airyscan (SR), QUASAR (spectral), T-PMT |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 445±5nm, Laser: 474±5 nm, Laser: 488±5 nm, Laser: 514±5nm, Laser: 640±5 nm, LED: 385-395nm, LED: 430-450nm, LED: 470-475nm, LED: 545-555nm, LED: 640-650nm, LED: 730-740nm |
| System_Specification | Illumination: Fluorescence, Illumination: Bightfield, Incubation: heating chamber, Incubation: CO2-gas mixer, Automated Stage, Z-piezo / galvo Stage, Focus Stabilization |
| Technology / Application | FCS / FCCS / Dynamic profiler, FRAP, FRET, Time-lapse recording, Multi-position, In-vivo imaging, Photoactivation / -conversion, Reflection Microscopy, Spectral Imaging, Spectral unmixing, SR: Airyscan (140nm), Super Resolution (SR) |
