Match pinhole option faulty in Zen 2.5

With the introduction of the new ZEN 2.5 software on our new LSM800 systems, we uncovered some things that do not work optimally for us. Most importantly, the ‘Match Pinholes’ does not work, and instead of matching the pinholes to create equal optical sections per wavelength, this option now opens the pinholes completely! Opening the pinhole might overexpose the detectors and damage them: Do not use the ‘match pinhole’ option in ZEN 2.5 when setting up your Z-stack until further notice. We are in contact with Zeiss and ope for a solution soon!