The Bioimaging Facility at IST Austria is hosting a demonstration of the ZEISS Axioscan Z1 slidescanner May until June, 2021. The setup will be available with full service for you – thereby enabling to compare both speed and acquisition time to your current workflows → see: How to get your slides scanned? (below)
Specification:
- 25 mounting frames for 4 slides (= 100 slides loading capacity in 1 time)
- Contrast techniques:
- TL Brightfield
- TL Ring Aperature Contrast (RAC)
- 7 solid state LED lamps (Kolibri 7):
- (630nm) for excitation of Cy5, Alexa 631, TOTO-3 and similar dyes
- Yellow (590nm) for excitation of mCherry, Alexa 568, mPlum and similar dyes
- Green (555nm) for excitation of Cy3, TRITC, DsRed and similar dyes
- Cyan (511nm) for excitation of eYFP, Eosin, TOTO-1 and similar dyes
- Blue (475nm) for excitation of eGFP, Fluo4, FITC and similar dyes
- Violet (430nm) for excitation of eCFP, Lucifer Yellow, Alexa 430 and similar dyes
- UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 33258 and similar dyes
Objectives:
- Fluar 5x/0.25 M27 (FWD=12.5mm)
- Plan-Apochromat 10x/0.45 M27 (FWD=2.1mm)
- Plan-Apochromat 20x/0.8 M27
- Plan-Apochromat 40x/0.95 Corr M27 (FWD=0.25mm)
Cameras:
- High-End Hitachi HV F202 color camera (histology)
- High-End monochrome Hamamatsu Orca Flash 4.0 V3 (fluorescence)
Modules:
- ZEN Module Macro Environment
- ZEN Module Image Analysis
- ZEN Module Advanced Processing & Analysis
- ZEN Module Intellesis
How to get your slides scanned? – free of charge for duration of the demo period!
- Please take note that we needs to be requested 48h in advance
- A first ‘Slide Scanning Request’, we will require an ‘assisted session’ to establish the ‘acquisition protocol’. This also accounts for any request with samples that significantly differ from previous established protocols!
1. Select the “order“-tab in PPMS → order
2. Select the quantity of “slide-scanner slides” you want to have imaged
3. Fill-out and submit the form, and bring your slides to the drop-off in our office in i06.EG.108
- It is possible to mark the sections with a marker pen to facilitate and speed up tissue detection
- Various Bar- and QR-Codes are supported for slide naming (see product info sheet)
4. After completion, you will get notified that your data has been copied to your scratch-folder and that your sampled can be collected again from our office in i06.EG.108
What are the advantages of using this system?
- Extremely fast
- Gentle illumination
- Automated sample acquisition workflows
- High throughput
More info about the system:
Download the product info pfd:
Product info Axio-Scan.Z1
ZEISS Homepage – Slide Scanner Axio Scan Z1
(Forwards to new Axio Scan 7)