The CytoFLEX 02 is a compact cell analyzer with two lasers and 6 color parameters that together facilitate a broad spectrum of applications. The spatially separated lasers allows panels to be spread across the spectrum reducing cross talk and spectral overlap.
Description
Location: Moonstone/i24, room I24.EG.015 Book in PPMS or Request a training
- software: CytExpert Software
- 2 lasers: 488 nm & 638 nm
- 6 channels for fluorescence detection
Available or repositionable band-pass filters:
- 405/10,
- 450/45
- 525/40 (2x), 1 assigned to FITC channel on 488 laser
- 585/42 assigned to PE channel
- 610/20 (2x)
- 638/6
- 660/10 (2x), 1 assigned to APC channel
- 690/50 assigned to PC5.5 channel
- 712/25 assigned to APC-A700 channel
- 780/60 (3x), 1 assigned to APC-A750 channel
System manual and additional material is available on SeaFile (login required).
Additional Information:
- This link can be used to design the experimental panel
- BC Conjugated Antibodies and Antibody Cocktails
- CytoFLEX Platform Instructions for Use
Contact for application and experiment planning:
- ticket: cytometry@ist.ac.at
Additional information
| Building | i24 |
|---|---|
| Fluorescent Lightsource | Laser: 488±5 nm, Laser: 640±5 nm |
| Camera / Detector | APDs (Avalanche Photodiodes) |
