The (Dual-) Pipette Aspiration (DPA) assay is a versatile tool that allows micro-rheology, force probing and the measurement of a range of biophysical parameters of cells and tissue by precise micro-manipulation of cells and tissues and soft materials, in combination with concurrent high-resolution imaging. Post-processing allows to determine properties such as contact-formation, de-adhesion force, surface tension, elasticity and viscosity of biological samples. More information:
Description
Movie 1: Time-lapse of an Pipette Aspiration Assay performed on the Yolk Syncytional Layer (YSL) of an early stage zebrafish embryo (red = YSL fluid; green: YSL nuclei) – 5 fps
Available on Leica Stellaris 5 inverted & Nikon CSU-W1-02 (see related systems for more details)
Learn more about the Pipette-based Aspiration Assay in this manuscript
Additional information
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