The idea behind the sample flipper is to temporarily convert any inverted microscope into a vertical microscope (more details):
The lights can only be used on the sample flipper compatible systems, after a training with facility staff → request a Training
Description
The Imaging and Optics facility “Automated Rental System” allows the flexibility for temporal usage of Experimental Accessories, Optical Tools, (high-end) Objectives. All items available need to be reserved in the booking system (PPMS – booking rights apply). Upon making a reservation, an access-code is generated for the duration of the reservation. For overnight reservations, a second access code is generated for the following day and sent by separate email.
The idea behind the sample flipper is to temporarily convert any inverted microscope into a vertical microscope (more details):
Between image acquisitions the sample chamber is turned vertically and the grow light is switched on. In this way the plants are in their normal, healthy orientation, with gravity pointing away from the leaves toward the roots and with light above the leaves. Only during imaging the entire chamber is rotated into a horizontal orientation and the plants can now be imaged in the normal inverted configuration with the grow light automatically being turned off.
Additionally, a pump that disperses one drop of immersion water before imaging is available. This works by having a flexible tube hanging down exactly over the front lens Once the chamber is flipped down for imaging the tube is automatically pushed out the way by the chamber.
The flipping of the chamber as well as the water dispersion is controlled by a separate program that is synchronized with the image acquisition.
Contact: Robert Hauschild (CZI-supported Staff scientist @ IOF / ISTA)
Additional information
| Building | i04 |
|---|---|
| System_Specification | Sample Flipper compatible (Rental item) |
