Zeiss Axio Observer (inverted)

Location (I06.U1.406)

Non-automated inverted Widefield microscope for quick checking of samples and ‘snap-shot” image capture options

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Description

Inverted fluorescence widefield Microscope

Specification

  • Manual stage
  • Light sources: halogen lamp (TL) + CoolLED p300 SB light (RL)
  • Detectors: Zeiss ICm1 monochrome CCD camera (1388×1038 Pixel))
  • Software: ZenBlue lite 3

Objectives (M27 thread):

  1. EC-Plan-NeoFluar 2.5x /0.075 AIR (420320-9901), WD = 9.5 mm
  2. Plan-Apochromat 5x /0.16 AIR (420630-9900), WD = 12.1 mm
  3. Plan-Apochromat 10x/0.45 AIR (420640-9900), WD =0.45 mm
  4. Plan-Apochromat 20x/0.4 AIR (420650-9901), WD = 0.55 mm (D=0.17 mm)
  5. LCI Plan Neofluar 63x/1.3 Water, Silicone oil and Glycerine (420882-9970-720), WD=0.17 mm

Filters

  1. DAPI – G365 ft395 bp445/50 (Zeiss, filter set 49)
  2. GFP – bp470/40, ft495, bp 525/50 (Zeiss, filter set 38)
  3. TxRed (F46-008, AHF)
  4. Fluolid Orange (Acridin Orange)
  5. Dualband GFP/DsRed (F56-420, AHF)
  6. Empty (TL)

Additional information

Building

i06

Camera / Detector

CCD

Fluorescent Lightsource

LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm

System_Specification

Illumination: Fluorescence, Illumination: Bightfield