Zeiss LSM-02 (800-inverted)

Description

Location: i24.U1.031-B2

Axio Imager.Z2 Inverted LSM800 confocal microscope + Airyscan, ZEN 3.8 with experiment designer and macro-environment (OAD)

This confocal microscope is well suited for life imaging: book in PPMS

Light sources:

• Laser lines 405, 488, 561, 640 nm
• CoolLED p300 SB light
• ESID LED

Detectors: 3 channels simultaneously (more sequentially)

• 2x GaAsP PMTs: Gallium arsenide phosphide photomultiplier tube detectors with free choice of the spectral range. (Uses a tuneable short-pass and band-pass filter in front of the PMTs)
• 1 x Airyscan detector – optimized for 63x. Can also be used as 3rd GaAsP detector!
• 1x ESID+LED light source: Electronically switchable illumination and detection module

Motorized stage for multiple position imaging

• Motorized stage for multiple position imaging
• Dish holder, single slide holder

Objectives (M27 thread)

1. Plan-Apochromat 10x/0.45 (420640-9900), WD=2.1 mm
2. Pla-Apochromat 20x/0.8 (420650-9901), WD=0.55 mm (D=0.17 mm)
3. Plan-Apochromat 40x/1.3 Oil DIC, UV-IR (420762-9800-799), WD=0.20 mm (Airyscan)
4. Objective Plan-Apochromat 63x / NA 1.4 Oil

Software: Zen 3.8

1. ZEN Module Experiment Designer Hardware
2. ZEN Module Tiles & Positions
3. ZEN Macro Editor

Computer: HP Z6

• Intel® Xeon™ E5-2623 v3 CPU @ 3.0GHz + 2GHz (2 processors)
• 64GB RAM
• 3.38TB data drive

Contact persons:

• IOF@ist.ac.at

System manual and additional material is available on SeaFile (login required).