Nikon Ti2E stand equipped with iLas2 (GATACA) 360° / Ring / Azimuthal TIRF module. This module is using a pair of fast galvanometers to be able to spin the laser in the back focal plane of the objective several times during a single exposure time. That way artifacts such as fringes or rings caused by laser interference patterns are averaged out.
Description
Location: I04.U1.019 (Bertalanffy Foundation Building)
Coming Soon…
System description and specifications
- 360° TIRF imaging
- FRAP (with all laser lines)
- Photo-activation / conversion (with all laser lines)
- Micro-patterning
- Widefield imaging
- Motorized stage
- Perfect focus system
- Laser angle sweep
Microscope and sample conditions
- Temperature controlled chamber
- CO2 supply
Light sources
- CoolLED pE-300 white MB LEDs for widefield fluorescence imaging and visual inspection of samples
- LED light source for transmitted light visual inspection of samples (+ GIF filter)
- Omicron LightHUB Ultra laser system for TIRF imaging & FRAP: 405 nm (120 mW), 488 nm (100 mW), 561 nm (100 mW), 640 nm (100 mW)
Cameras
- 2 x Photometrics Kinetix 22 mm sCMOS camera 2400 x 2400 active pixels, pixel size 6.5 μm x 6.5 μm, sensor area: 15.6 mm x 15.6 mm; 22.1 mm diagonal
- Digital Sight 100 front illuminated CMOS colour camera for back focal plane imaging, 4864 x 3648 pixels, pixel size 2.4 µm
Objectives
- 20x / NA 0.8 / WD 0.80 mm CFI Plan Apochromat lambda D (MRD70270)
- 60x / NA 1.49 / WD 0.13 mm oil CFI Apochromat TIRF, with correction collar for coverglass thickness (0.13-0.20 mm), chromatic correction 435-1064 nm (MRD01691)
- 100x / NA 1.49 / WD 0.12 mm oil CFI Apochromat TIRF, with correction collar for coverglass thickness (0.13–0.20 mm), chromatic correction 435–1064 nm (MRD01991)
Dual-Cam filter option
CAIRN TwinCam camera splitter (0.76x magnification)
- TwinCam cube dichroic mirror red/far red 643 nm
- TwinCam cube dichroic mirror green/red,far red: 560 nm
- TwinCam cube dichroic mirror blue/green: 488 nm (on request)
Bandpassfilters for TwinCam
- 450/50 ET bandpass
- 525/50 ET bandpass
- 595/50 ET bandpass
- 635 longpass filter (transmission 660 – 1200 nm)
- 700/75 ET bandpass
Filtercubes for Epifluorescence
- LED-DAPI-B filter cube Ex 392/23, Em 447/60
- LED-FITC-A filter cube Ex 474/27, Em 525/45
- LED-TRITC-A filter cube Ex 554/23, Em 609/54
- LED-Cy5-A filter cube Ex 635/18, Em 680/42
Software
NIS-Elements AR 6.20.03
Computer: HP Z4 G5 workstation
- Intel Xeon W3 4.3 GHz 8 Cores
- RAM:128 GB DDR5
- 512 GB OS Drive
- 2x 2 TB SSD Fast Storage
- 8 TB Data Storage
- NVIDIA Quadro RTX4000 (20GB)
System manual and additional material is available on SeaFile (login required).
Additional information
| Building | i06 |
|---|---|
| Camera / Detector | sCMOS |
| Fluorescent Lightsource | Laser: 405±5 nm, Laser: 488±5 nm, Laser: 561±5 nm, Laser: 640±5 nm, LED: 355-365nm, LED: 430-450nm, LED: 545-555nm, LED: 640-650nm |
| System_Specification | Illumination: Fluorescence, Illumination: DIC, Incubation: heating chamber, Incubation: CO2/N2-gas mixer (hypoxia), Automated Stage, Z-piezo / galvo Stage, Focus Stabilization, Dual Camera (see detectors) |
| Technology / Application | 360° / Ring / Azimuthal TIRF, FRAP, In-vivo imaging, Micro-patterning, Photoactivation / -conversion, SAIM, SR: SMLM (20nm) |
