Miltenyi MACSQuant Tyto Lux

Description

Available Laser excitation:

• 405 nm, 100 mW
• 488 nm, 100 mW
• 552 nm, 100 mW
• 638 nm, 100 mW

(The 488nm and 552nm laser lines share an interrogation spot, 405nm and 638nm are separate)

 

Available Emission detectors:

• Backscatter and side scatter for detection of size & granularity of cells
• V1: 448/20 nm
• V2: 520/36 nm
• V3: 579/34 nm
• V4: 641/75 nm
• B1: 520/36 nm
• B/Y2: 579/34 nm
• B/Y3: 615/20 nm
• B/Y4: 679/75 nm
• B/Y5: 785/62 nm
• R1: 667/30 nm
• R2: 725/40 nm
• R3: 785/62 nm

 

Cell flow rate:

• Up to 55,000 cells/s for the MACSQuant Tyto Cartridge
• up to 110,000 cells/s for the MACSQuant Tyto Cartridge HS
• Sort rate Up to 30,000 valve actuations/s

 

Performance:

• Fluorescence performance 5 decade logarithmic scales, display in lin, log, or hlog scales
• Purity >99%*
• Viability >99% for lymphocytes*
• Sorting mode Two-way sorting of sorted and non-sorted cells
• Operating pressure <230 mbar (3 psi) with the MACSQuant Tyto Cartridge and 1100 mbar (<14 psi)
  with the MACSQuant Tyto Cartridge HS
• Temperature control range 4–25 °C (under standard environmental conditions)
• Carry-over No sample-to-sample carry-over due to single-use disposable cartridge
• Data files .mqd (proprietary file type); fcs (2.0, 3.0, 3.1 compatible)

 

Cartridge details:

• Sterility Ethylene oxide–validated sterilization method to guarantee sterility of each cartridge
• A-septic filters 0.1 µm hydrophobic filters at air inlet and outlet ports
• Sorting channel width 25 × 50 µm
• Maximum loading volume (input) 100 µL–10 mL
• Maximum loading cell number 5×107 cells/mL
• Cell retrieval No cell loss – negatively sorted cells are kept in the negative collection chamber
• Barcode labeling Cartridges are labeled with a barcode for easy identification and traceability

 

The Manual and additional information are available on SeaFile.